Sedative Effects of Intraperitoneal Diazepam in Mice

Authors

  • Marsha Vania Faculty of Medicine, Universitas Muhammadiyah Prof. DR. HAMKA
  • Shinta Dewi Permata Sari Faculty of Medicine, Universitas Muhammadiyah Prof. DR. HAMKA https://orcid.org/0000-0002-8993-5878 (unauthenticated)
  • Bambang Siswitono Faculty of Medicine, Universitas Muhammadiyah Prof. DR. HAMKA
  • Endin Nokik Stujanna Faculty of Medicine, Universitas Muhammadiyah Prof. DR. HAMKA

Keywords:

Diazepam, Fireplace Test, Phenobarbital, Traction Test.

Abstract

Objectives: To determine the effectiveness of Diazepam in comparison with Phenobarbital.

Methods: Twenty-seven male Swiss Webster mice were used and randomly divided into three groups of negative control (NS), positive control (Phenobarbital), and diazepam group. Two tests were performed on these group: the traction Test and the Fireplace Test. Pupillary diameter was also observed.

Results: A significant difference based on the Kruskal - Wallis statistical test was observed between the positive control and the diazepam group (<0.05) in the traction test, which was also true for the fireplaced test (p<0.05). The pupillary diameter in the test animals in the positive control and diazepam group was not statistically significant (p>0.05).

Conclusion: Diazepam has a better sedative effect than Phenobarbital. The sedative effect produced by Diazepam is stronger, with faster onset and longer half-life than the Phenobarbital the positive control. However, different test methods and comparisons should be sought to support this conclusion.

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Published

2023-03-31

Issue

Vol. 11 No. 1 (2023)

Section

Articles

License

Authors retain copyright and grant International Journal of Integrated Health Sciences right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to freely share and remix the work with an acknowledgement of the work's authorship and initial publication in International Journal of Integrated Health Sciences.