Acute and Sub-chronic Toxicity Studies of Combination of Physalis angulata L. (Cecendet) Extract and Methylprednisolone on Animals
DOI:
https://doi.org/10.15850/ijihs.v7n1.1619Keywords:
acute toxicity, LD50, Physallis angulata L, Lupus, subchronic toxicityAbstract
Objective: To evaluate the safety of combination of Physalis angulata L. (Cecendet) extract and methylprednisolone through a single dose in mice (acute toxicity) and repeated doses within 90 days in rats (subchronic toxicity).
Methods: Acute toxicity test was conducted to 2 groups of female mice by monitoring their behavior and death at 4 hours and 24 hours after cecendet adminstration. While subchronic toxicity test was conducted to 6 groups of rats as normal group, control group, and receive varoius cecendet dose. Each group was combined with methylprednisolone 5 mg/kg b.w. at first month and gradually taperred to 3.2 mg/kg b.w. at third month. Subchronic toxicity was evaluated by monitoring rats’ behavior, mortality, blood biochemistry, blood cell count, urinalysis, and organ macroscopic and histologic.
Results: The study showed cecendet extracts did not affect mice behavior in single dose therapy up to 5 g/kg b.w. and had LD50 of more than 5 g/kg b.w., which was categorized as practically non-toxic. The sub-chronic toxicity study showed that up to 1 g/kg b.w. of cecendet extracts that was administered for 90 days did not cause death, was not organ toxic, did not affect blood biochemistries, blood cell count, and urinalysis.
Conclusions: Cecendet extract and methylprednisolone combination was safe based on acute and sub-chronic toxicity data.
Keywords: Acute toxicity, LD50, Lupus, Physalis angulata L, subchronic
toxicity
References
Pons GJ, Alarcón GS, Lacie S. Understanding the epidemiology and progression of Systemic Lupus Erythematosus. Semin Arthritis Rheum. 2010;39(4):257–68.
Perhimpunan Reumatologi Indonesia. Rekomendasi Perhimpunan Reumatologi Indonesia untuk diagnosis dan pengelolaan Lupus Eritematosus Sistemik. Jakarta: Perhimpunan Reumatologi Indonesia; 2011.
Chaidir L, Epi, Taofik A. Eksplorasi, identifikasi, dan perbanyakan tanaman ciplukan (Physalis angulata) dengan menggunakan metode generatif dan vegetatif. J Ilmu Pertanian. 2015;9(1):82–90.
Yang YJ, Yi L, Wang Q, Xie BB, Dong Y, Sha CW. Anti-inflammatory effects of physalin E from Physalis angulata on lipopolysaccharide-stimulated RAW 264.7 cells through inhibition of NF-κB pathway, Immunopharmacol Immunotoxicol. 2017;39(2):74–9.
Sun L, Liu J, Liu P, Yu Y, Ma L, Hu L. Immunosuppression effect of Withangulatin A from Physalis angulata via heme oxygenase 1-dependent pathways. Process Biochem 2011;46(2):482–8.
Adnyana IK, Sukandar EY, Maeistuti N, Setiawan F. Evaluation of ethanolic extracts of mullaca (Physallis angulata L.) herbs for treatment of lupus disease in mice induced pristane. Procedia Chemistry. 2014;13(2014);186–93.
SN Vikasari, AB Sutjiatmo, EY Sukandar, S Suryani, PA Perdana. Efek ekstrak etanol herba cecendet (Physalis angulata L.) pada kadar proteinuria hewan model lupus eritematosus sistemik. Kartika J Ilmiah Farm. 2014;2(1):15–9.
Wang ZL, Luo XF, Li MT, Xu D, Zhou S, Chen HZ. Resveratrol possesses protective effects in a pristane-induced lupus mouse model. PLoS One [serial on the internet]. 2014 Dec [cited 2019 Jan 17];9(12):[about 16p.]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263676/.
Badan Pengawas Obat dan Makanan. Peraturan Kepala Badan Pengawas Obat dan makanan Republik Indonesia Nomor 875 tahun 2014 tentang pedoman uji toksisitas nonklinik secara invivo. Jakarta: Badan Pengawas Obat dan Makanan; 2014.
Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AA, Vernekar SN. Markers of renal function tests. N Am J Med Sci. 2010;2(4):170–3.
Sharma A, Hirulkar NB, Wadel P, Das P. Influence of Hyperglycemia on Renal Function Parameter in Patients with DiabeteS Mellitus. IJPBA 2011;2(Suppl 2):734–9.
Arthur FKN, Woode E, Terlabi EO, Larbie C. Evaluation of acute and subchronic toxicity of Annona Muricata (Linn.) aqueous extract in animals. Eur J Exp Biol. 2011;1(Suppl 4):115–24.
Downloads
Published
Issue
Section
License
Authors retain copyright and grant International Journal of Integrated Health Sciences right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to freely share and remix the work with an acknowledgement of the work's authorship and initial publication in International Journal of Integrated Health Sciences.
