Effect of Vitamin D Supplementation on Irisin, Telomerase, Klotho, and Tumor Necrosis Factor-alpha (TNF-α) in Elderly: A Quasi-Experimental Study

Authors

  • Diniwati Mukhtar Department of Physiology, Faculty of Medicine, YARSI University
  • Nunung Ainur Rahmah Department of Anatomical Pathology, Faculty of Medicine, YARSI University, Jakarta
  • Sri Wuryanti Department of Clinical Nutrition, Faculty of Medicine, YARSI University
  • Himmi Marsiati Department of Medical Education, Faculty of Medicine, YARSI University and Department of Biomedical Science Doctoral, Graduate School, YARSI University
  • Desi Ariyani Haddad Doctoral Candidate, Department of Biomedical Science Doctoral, Graduate School, YARSI University
  • Sabarina Elfrida Manik Doctoral Program in Biomedical Sciences, Graduate School, YARSI University Jakarta
  • Linda Weni Department of Biomedical Science Doctoral, Graduate School, YARSI University, Jakarta
  • Bistamy Muhammad Nursabur Faculty of Medicine Universitas Padjadjaran
  • Andrea Ivana Allicia Hutabarat Faculty of Medicine Universitas Padjadjaran https://orcid.org/0009-0008-6440-3294 (unauthenticated)
  • Switania Putri Puspita Akademi Teknologi Maritim Patimban Subang

DOI:

https://doi.org/10.15850/amj.v12n3.4286

Keywords:

Aging, irisin, klotho, telomerase, TNF-α, vitamin D

Abstract

Background: Vitamin D plays a crucial role in aging by regulating mitochondrial function, inflammation, oxidative stress, and telomere stability. Vitamin D deficiency is common among the elderly and is linked to accelerated aging. Biomarkers such as irisin, telomerase, klotho, and tumor necrosis factor-alpha (TNF-α) are associated with aging processes. This study aimed to evaluate the effect of vitamin D supplementation on these biomarkers in elderly individuals.

Methods: This quasi-experimental pretest-posttest study was conducted in Kadugadung Village, Banten, Indonesia from Maret to September 2024.  A total of 47 healthy elderly individuals (≥60 years) were recruited using purposive sampling. The treatment group (n=25) received 800 IU/day of vitamin D for 20 days, whereas the control group (n=22) received none. Blood samples were collected before and after the intervention to measure serum irisin, telomerase activity, klotho, and TNF-α. Baseline variables included body mass index (BMI), blood pressure, hemoglobin, hematocrit, blood glucose, cholesterol, and uric acid. Data were analyzed using paired and independent statistical tests.

Results: Vitamin D supplementation significantly increased serum irisin levels (p=0.016), meanwhile no significant changes were observed in telomerase activity (p=0.128), klotho (p=0.819), or TNF-α (p=0.098). In the treatment group, blood glucose was correlated positively with TNF-α (r=0.423, p<0.05), whereas cholesterol was correlated negatively with TNF-α (r=-0.51, p<0.01). Furthermore, telomerase activity was correlated positively with irisin (r=0.348, p<0.05)

Conclusion: Vitamin D supplementation at 800 IU/day significantly enhances serum irisin, but does not affect telomerase, klotho, or TNF-α. These findings suggest a potential role of vitamin D in modulating aging-related biomarkers.

 

Correction Notice: This article has been corrected. See the Erratum published in Althea Medical Journal, Volume 12, Number 4 (2025).

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Published

2025-09-30

Issue

Vol. 12 No. 3 (2025)

Section

Articles

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