Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay

Authors

  • Nayla Majeda Alfarafisa Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran
  • Astrid Feinisa Khairani Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran https://orcid.org/0000-0001-7242-4791 (unauthenticated)
  • Achadiyani Achadiyani Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran
  • Yoan Chou Master Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran
  • Fitri Aulia Master Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran
  • Muhammad Bintang Firdaus Faculty of Medicine Universitas Padjadjaran

DOI:

https://doi.org/10.15850/amj.v12n2.3954

Keywords:

Alpha-tocopheryl acetate, anti-aging, cell viability, metformin hydrocholride, MTT assay

Abstract

Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human dermal fibroblasts (HDFs), a primary cell type in skin aging research.

Methods: HDFs were isolated using a mechanical isolation method and cultured under standard conditions. Cells were treated with varying concentrations of alpha-tocopheryl acetate and metformin hydrochloride as independents agents. After 48 hours of incubation, cell viability was measured using the MTT assay.

Results: Alpha-tocopheryl acetate had the highest HDF cell viability (107%) at a concentration of 50 μM. Metformin hydrochloride had the maximum HDF cell viability (158%) at 5 μM. However, the viability response varied across different concentrations for both agents, indicating that optimal dosing was essential for maximizing their effectiveness.

Conclusions: Alpha-tocopheryl acetate at 50 μM and metformin hydrochloride at 50 μM yield the highest viability of HDFs in vitro. These findings suggest potential roles for both agents in anti-aging skin therapies. Further research is recommended to explore their mechanisms of action and to optimize dosing strategies for clinical applications.

Author Biographies

  • Achadiyani Achadiyani, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran
    Department of Biomedical Sciences, Division of Cell Biology
  • Fitri Aulia, Master Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran
    Graduate School of Master Program in Anti-aging and Aesthetic Medicine
  • Muhammad Bintang Firdaus, Faculty of Medicine Universitas Padjadjaran
    Medical program

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Published

2025-06-27

Issue

Vol. 12 No. 2 (2025)

Section

Articles

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